Our Reagents

Antibodies

Available volume discounts and contract testing services.

Anti-Perilipin 1 Antibody (Cat. 4854)

$ 429.00 | 100 µL

Lipid droplets are protein-coated structures that regulate growth and metabolism. In adipocytes, perilipin is the key associated protein. Mice lacking perilipin exhibit a lean phenotype due to reduced fat accumulation, suggesting that perilipin functions as a gatekeeper, shielding triglycerides from lipase-mediated metabolism.

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Target: Full-length perilipin 1 (PLIN1a)
Reactivity: Human, mouse (verified)
Applications: ICC/IF, WB
Dilutions: ICC/IF 1:300 | WB 1:5000
Immunogen: Synthetic peptide near serine 497 of human perilipin 1 (not phosphorylation-specific)

Human subcutaneous adipocytes stained for nuclei (blue), lipid droplets (green), and perilipin (red) using Vala Sciences’ lipid droplet staining kit (#4805) and mouse monoclonal antibody to perilipin (# 4854).

4°C short-term; -20°C long-term

Anti-P-Perilipin 1-Serine 497 Antibody (Cat. 4855)

$ 549.00 | 100 µL

Lipid droplets are protein-coated structures that regulate growth and metabolism. Perilipin, the key adipocyte-associated protein, acts as a gatekeeper, protecting triglycerides from lipase-mediated metabolism. Perilipin knockout mice exhibit reduced fat accumulation. Upon lipolysis activation, PKA phosphorylates perilipin at Ser497 and Ser522, facilitating HSL recruitment to lipid droplets.

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Target: Phospho-Ser497 (human), Phospho-Ser492 (mouse)
Reactivity: Human, mouse (verified)
Applications: ICC/IF
Dilutions: ICC/IF (1:100)
Immunogen: Synthetic peptide near Ser497 of human perilipin 1

Human subcutaneous adipocytes were incubated without (control, left) or with (right) the PKA activator forskolin. Cells were stained for nuclei (blue), lipid droplets (green), and phospho-perilipin (Ser497) (red) using Vala Sciences’ Lipid Droplet Staining Kit (#4805) and mouse monoclonal phospho-perilipin (Ser497) antibody (#4855).

4°C short-term; -20°C long-term

Anti-P-Perilipin 1-Serine 522 Antibody (Cat. 4856)

$ 549.00 | 100 µL

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Target: Phospho-Ser522 (human), Phospho-Ser517 (mouse)
Reactivity: Human, mouse (verified)
Applications: ICC/IF, WB
Dilutions: ICC/IF 1:30 | WB 1:5000
Immunogen: Synthetic peptide near Ser522 of human perilipin 1

Human subcutaneous adipocytes were incubated in the presence and absence of the lipolytic activator Lys-3γ-MSH prior to being stained for nuclei (blue), lipid droplets (green), and phospho-perilipin (Ser 522) (red) using Vala Sciences’ lipid droplet staining kit (#4805) and mouse monoclonal antibody to phospho-perilipin (Ser 522) (# 4856).

4°C short-term; -20°C long-term

Reagents

Available volume discounts and contract testing services.

Lipid Droplet Screen-Certified Kit (Cat. 4805)

$ 329.00

Lipid droplets are the primary fat storage organelles of fat cells (adipocytes). Lipid droplet size, and number are altered by anti-diabetic and anti-obesity drugs and these effects can be quantified utilizing Vala Science Inc’s Lipid Droplet Kit. The kit can be utilized to quantify lipid droplets in human primary adipocytes, murine 3T3L1 adipocytes, as well as primary or immortalized hepatocytes.

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Reagent A: Fixative 60 ml
Reagent B: Permeabilizer 60 ml
Reagent C: Lipid stain 18 ml base solution
Reagent D: Nuclear stain 60 ml

Protocol Summary

Fix Cells: Rinse with cold PBS, add 100 µl Reagent A, incubate 15 min at RT, rinse with PBS.
Permeabilize: Add 100 µl Reagent B, incubate 15 min at 37°C with rotation, then remove.
Stain Lipid Droplets: Dilute Reagent C (3 µl per ml base solution), add 100 µl/well, incubate 60 min at 37°C with rotation, rinse 3X with PBS.
Stain Nuclei: Add 100 µl Reagent D, incubate 20 min at RT.
Ready for Imaging.

Sufficient for five 96-well dishes

Control Cell Extract, 3t3l1 Adipocytes (Cat. 4860)

$ 159 | 100 µg

This cell extract serves as a control for Western blotting procedures (e.g., for use with Anti-p-Perilipin 1-serine 522, Cat. 4856); the extract was derived from cells that were not exposed to lipolysis-activating agents.

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Concentration: 2 mg/mL

-20°C short-term; -80°C long-term

Forskolin-Treated Cell Extract, 3t3l1 Adipocytes (Cat. 4861)

$ 219 | 100 µg

This cell extract serves as a control for Western blotting procedures (e.g., for use with Anti-p-Perilipin 1-serine 522, Cat. 4856); the extract was derived from cells exposed to 6 µM forskolin for 20 minutes, in order to activate lipolysis.

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Concentration: 2 mg/mL

-20°C short-term; -80°C long-term

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Purchase Orders

Vala Sciences accepts Purchase Orders (POs) from qualified buyers. If your company or institution is not yet qualified, please contact us using the information below.

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